Frequently Asked Questions
NIF calculator (See GENEie paper: doi.org/TBC) is a simple online tool where you can paste a nucleotide sequence and easily calculate the Donor and Acceptor motif NIF values.
A splice-site can be divided into overlapping windows of 9-nucleotides (nt) to measure splicesite strength based on how commonly the 9-nt sequence occurs at a given position of the exon-intron junction among all annotated human splice-sites; A measure defined as Native Intron Frequency or NIF. (GENEie paper: doi.org/TBC)